Rivera to Perform “Valerie” on the 100th Episode of “Glee”

Naya Rivera is preparing to reinterpret one of her most memorable Glee performances…

The 27-year-old half-Puerto Rican actress/singer will offer a new take on her acclaimed performance of Amy Winehouse and Mark Ronson’s version of “Valerie” for the Fox musical dramedy’s 100th episode.

Naya Rivera

In the second season episode entitled “Special Education,” Rivera sang lead on the track by The Zutons during the New Directions’ performance at the 2010 Western Ohio Sectional Championship.

Rivera also performed the track in the Glee: The 3D Concert Movie, which was released in 2011.

This time around, she’ll perform the song alongside Heather Morris (Brittany).

In addition, Rivera will also perform Britney Spears’ “Toxic” with Morris and Diana Agron (Quinn), and “Be Okay” with Lea Michele.

The music in the two-part episode was chosen by the show’s fans, who were asked to pick 10 of the show’s 30 most beloved performances to be remixed and featured on the series’ landmark episode.

Set to air on March 18 and 25 at 8 p.m., the 100th episode will center on Jane Lynch‘s principal Sue Sylvester, who after years of disdain for the New Directions and Matthew Morrison‘s Will Schuester, finally succeeds in ending the glee club in Lima.

Many original castmembers — Morris (Brittany), Harry Shum Jr. (Mike), Mark Salling (Puck), Dancing With the Stars winner Amber Riley (Mercedes), Agron (Quinn) as well as Kristin Chenoweth (April) and Gwyneth Paltrow (Holly Holliday) — will return to say their final goodbyes to Will and each other in the emotional episode.

To honor the milestone, Columbia Records will release Glee: The Music Celebrating 100 Episodes, which will include brand-new versions of Glee favorites from the original cast — including Journey‘s “Don’t Stop Believin’,” Pink‘s “Raise Your Glass” and the original song “Loser Like Me.”

The album will be released on March 25.

Here’s the complete 100th Glee tracklist:

1. “Keep Holding On”* featuring Mark Salling (Puck)
2. “Valerie”* featuring Naya Rivera (Santana) and Morris (Brittany)
3. “Defying Gravity”* featuring Lea Michele( Rachel), Chris Colfer (Kurt) and Riley (Mercedes)
4. “Raise Your Glass”* featuring Morrison (Will) and Chenoweth (April)
5. “Toxic”* featuring Rivera (Santana), Morris (Brittany) and Agron (Quinn)
6. “Happy” featuring Paltrow (Holly), Chenoweth (April), Morrison (Will), Darren Criss(Blaine) and Riley (Mercedes)
7. “Party All the Time” featuring Paltrow (Holly)
8. “Total Eclipse of the Heart”* featuring Morrison (Will) and Chenoweth (April)
9. “Loser Like Me”* featuring Criss (Blaine), Kevin McHale (Artie), Chord Overstreet(Sam) and Jenna Ushkowitz (Tina)
10. “Be Okay” featuring Michele (Rachel) and Rivera (Santana)
11. “I Am Changing” featuring Riley (Mercedes) and Colfer (Kurt)
12. “Just Give Me a Reason” featuring Agron (Quinn) and Salling (Puck)
13. “Don’t Stop Believin’”* featuring Michele (Rachel), Criss (Blaine), Colfer (Kurt), Morrison (Will), McHale (Artie) and Ushkowitz (Tina)

* Season-five version

Glee returns from its midseason break on February 25 in its new night and time on Tuesdays at 8 p.m. on Fox.